A short synthetic chimeric sequence harboring matrix attachment region/PSAR2 increases transgene expression in Chinese hamster ovary cells. Issue 9 (2nd September 2017)

• Record Type: Journal Article
• Authors: Li, Qin; Wang, Wen; Guo, Xiao; Jia, Yan-Long; Wang, Yan-Fang; Wang, Tian-Yun
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 81:Issue 9(2017)
• Page Start: 1755
• Page End: 1761
• Publication Date: 2017-09-02
• Subjects: CHO cell -- matrix attachment regions -- transgene expression; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2017.1350563
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: A chimeric DNA fragment containing an interferon-beta matrix attachment region (MAR) and an immunoglobulin MAR (PSAR2) was synthesized. PSAR2 was cloned into the upstream or downstream region of an enhanced green fluorescent protein (eGFP) expression cassette in a eukaryotic vector, which was then transfected into CHO cells. The results showed that PSAR2 did not effectively increase transgene expression when it was cloned into the upstream region of the eGFP expression cassette. However, when inserted downstream of the eGFP expression cassette, PSAR2-enhanced transient transgene expression and significantly increased the numbers of stably transfected cells compared with the control vector. Additionally, PSAR2 significantly increased eGFP copy numbers as compared with the control vector. PSAR2 could significantly enhance transgene expression in CHO cells according to the position in the vector and increased transgene copy numbers. We found a short chimeric sequence harboring two MARs effectively increased transgene expression in CHO cells. Abstract : : PSAR2 could significantly enhance transgene expression in CHO cells according to the position in the vector and increased transgene copy numbers.