CE–MS-based metabolomics reveals the metabolic profile of maitake mushroom (Grifola frondosa) strains with different cultivation characteristics. Issue 12 (2nd December 2017)

• Record Type: Journal Article
• Authors: Sato, Mayumi; Miyagi, Atsuko; Yoneyama, Shozo; Gisusi, Seiki; Tokuji, Yoshihiko; Kawai-Yamada, Maki
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 81:Issue 12(2017)
• Page Start: 2314
• Page End: 2322
• Publication Date: 2017-12-02
• Subjects: Grifola frondosa -- metabolite profile -- capillary electrophoresis—mass spectrometry -- amino acid -- organic acid; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2017.1387049
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Maitake mushroom ( Grifola frondosa [Dicks.] Gray) is generally cultured using the sawdust of broadleaf trees. The maitake strain Gf433 has high production efficiency, with high-quality of fruiting bodies even when 30% of the birch sawdust on the basal substrate is replaced with conifer sawdust. We performed metabolome analysis to investigate the effect of different cultivation components on the metabolism of Gf433 and Mori52 by performing CE–MS on their fruiting bodies in different cultivation conditions to quantify the levels of amino acids, organic acids, and phosphorylated organic acids. We found that amino acid and organic acid content in Gf433 were not affected by the kind of sawdust. However, Gf433 contained more organic acids and less amino acids than Mori52, and Gf433 also contained more chitin compared with Mori52. We believe that these differences in the metabolome contents of the two strains are related to the high production efficiency of Gf433. Abstract: : The yield and the production efficiency of Maitake Gf433 (GL, GB) are higher than the existing strain Mori52 (MB). Gf433 contained more organic acids and less amino acids than MB.