Immunocytochemical detection of rhamnogalacturonan II on forming cell plates in cultured tobacco cells. Issue 5 (4th May 2017)

• Record Type: Journal Article
• Authors: Zhou, Ye; Awano, Tatsuya; Kobayashi, Masaru; Matoh, Toru; Takabe, Keiji
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 81:Issue 5(2017)
• Page Start: 899
• Page End: 905
• Publication Date: 2017-05-04
• Subjects: antibody -- cell plate -- Nicotiana tabacum -- pectin -- rhamnogalacturonan II; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2016.1270740
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Rhamnogalacturonan II (RG-II) is a region of pectin macromolecules that is present in plant primary cell walls. The RG-II region serves as the site of borate cross-linking within pectin, via which pectin macromolecules link together to form a gel. In this study, we examined whether RG-II is present in the cell plate, the precursor of primary cell walls that forms during cytokinesis. A structure inside dividing cells was labeled with a rabbit polyclonal anti-RG-II antibody and detected by immunofluorescence microscopy. An antibody against callose, a marker polysaccharide for the cell plate, also labeled the structure. In immunoelectron microscopy analyses using the anti-RG-II antibody, gold particles were distributed in electron-lucent vesicular structures that appeared to correspond to the forming cell plates in late anaphase cells. Together, these results suggest that RG-II is present in cell plates from the early phase of their assembly. Abstract : :