Identification of a mutation causing a defective spindle assembly checkpoint in high ethyl caproate-producing sake yeast strain K1801. Issue 8 (2nd August 2016)

• Record Type: Journal Article
• Authors: Goshima, Tetsuya; Nakamura, Ryo; Kume, Kazunori; Okada, Hiroki; Ichikawa, Eri; Tamura, Hiroyasu; Hasuda, Hirokazu; Inahashi, Masaaki; Okazaki, Naoto; Akao, Takeshi; Shimoi, Hitoshi; Mizunuma, Masaki; Ohya, Yoshikazu; Hirata, Dai
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 8(2016)
• Page Start: 1657
• Page End: 1662
• Publication Date: 2016-08-02
• Subjects: cerulenin-resistant -- checkpoint -- ethyl caproate -- morphological variation -- sake yeast; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2016.1184963
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: In high-quality sake brewing, the cerulenin-resistant sake yeast K1801 with high ethyl caproate-producing ability has been used widely; however, K1801 has a defective spindle assembly checkpoint (SAC). To identify the mutation causing this defect, we first searched for sake yeasts with a SAC-defect like K1801 and found that K13 had such a defect. Then, we searched for a common SNP in only K1801 and K13 by examining 15 checkpoint-related genes in 23 sake yeasts, and found 1 mutation, R48P of Cdc55, the PP2A regulatory B subunit that is important for the SAC. Furthermore, we confirmed that the Cdc55-R48P mutation was responsible for the SAC-defect in K1801 by molecular genetic analyses. Morphological analysis indicated that this mutation caused a high cell morphological variation. But this mutation did not affect the excellent brewing properties of K1801. Thus, this mutation is a target for breeding of a new risk-free K1801 with normal checkpoint integrity. Graphical abstract: : K1801 (triploid) having 2 copies of R48P-type cdc55 and 1 copy of R48-type cdc55 showed a defective spindle assembly checkpoint (SAC). Cdc55-R48P is semidominant mutation in the SAC function.