Investigating tumor perfusion by hyperpolarized 13C MRI with comparison to conventional gadolinium contrast‐enhanced MRI and pathology in orthotopic human GBM xenografts. Issue 2 (19th February 2016)

• Record Type: Journal Article
• Authors: Park, Ilwoo; von Morze, Cornelius; Lupo, Janine M.; Ardenkjaer‐Larsen, Jan H.; Kadambi, Achuta; Vigneron, Daniel B.; Nelson, Sarah J.
• Is Part Of: Magnetic resonance in medicine. Volume 77:Issue 2(2017)
• Page Start: 841
• Page End: 847
• Publication Date: 2016-02-19
• Subjects: dynamic nuclear polarization -- hyperpolarized 13C perfusion MRI -- brain tumor -- gadolinium dynamic susceptibility contrast imaging; Nuclear magnetic resonance -- Periodicals; Electron paramagnetic resonance -- Periodicals; 616.07548
• DOI: 10.1002/mrm.26155
• Languages: English
• ISSNs: 0740-3194

Abstract

Abstract : Purpose: Dissolution dynamic nuclear polarization (DNP) enables the acquisition of 13 C magnetic resonance data with a high sensitivity. Recently, metabolically inactive hyperpolarized 13 C‐labeled compounds have shown to be potentially useful for perfusion imaging. The purpose of this study was to validate hyperpolarized perfusion imaging methods by comparing with conventional gadolinium (Gd)‐based perfusion MRI techniques and pathology. Methods: Dynamic 13 C data using metabolically inactive hyperpolarized bis‐1, 1‐(hydroxymethyl)‐[1‐ 13 C]cyclopropane‐d8 (HMCP) were obtained from an orthotopic human glioblastoma (GBM) model for the characterization of tumor perfusion and compared with standard Gd‐based dynamic susceptibility contrast (DSC) MRI data and immunohistochemical analysis from resected brains. Results: Distinct HMCP perfusion characteristics were observed within the GBM tumors compared with contralateral normal brain tissue. The perfusion parameters obtained from the hyperpolarized HMCP data in tumor were strongly correlated with normalized peak height measured from the DSC images. The results from immunohistochemical analysis supported these findings by showing a high level of vascular staining for tumor that exhibited high levels of hyperpolarized HMCP signal. Conclusion: The results from this study have demonstrated that hyperpolarized HMCP data can be used as an indicator of tumor perfusion in an orthotopic xenograft model for GBM. Magn Reson Med 77:841–847, 2017. © 2016 International Society for Magnetic Resonance in Medicine