Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon. Issue 7 (3rd July 2018)

• Record Type: Journal Article
• Authors: Kishida, Kouhei; Ogawa, Natsumi; Ichihashi, Eikichi; Kato, Hiromi; Nagata, Yuji; Ohtsubo, Yoshiyuki; Tsuda, Masataka
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 82:Issue 7(2018)
• Page Start: 1169
• Page End: 1171
• Publication Date: 2018-07-03
• Subjects: Mycobacterium -- vector plasmid -- gene disruption -- PAH -- microbial degradation; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2018.1445522
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.