Neuroprotective effect of Citrus kawachiensis (Kawachi Bankan) peels, a rich source of naringin, against global cerebral ischemia/reperfusion injury in mice. Issue 7 (3rd July 2018)

• Record Type: Journal Article
• Authors: Okuyama, Satoshi; Yamamoto, Kana; Mori, Hirotomo; Sawamoto, Atsushi; Amakura, Yoshiaki; Yoshimura, Morio; Tamanaha, Arisa; Ohkubo, Yuu; Sugawara, Kuniaki; Sudo, Masahiko; Nakajima, Mitsunari; Furukawa, Yoshiko
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 82:Issue 7(2018)
• Page Start: 1216
• Page End: 1224
• Publication Date: 2018-07-03
• Subjects: Ischemic brain injury -- naringin -- Citrus kawachiensis -- anti-inflammation -- anti-oxidation; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2018.1456320
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Cerebral ischemia/reperfusion is known to induce the generation of reactive oxygen species and inflammatory responses. Numerous studies have demonstrated that naringin (NGIN) has anti-oxidant and anti-inflammatory properties. We previously reported that Citrus kawachiensis contains a large quantity of NGIN in its peel. In the present study, we orally ( p.o. ) administered dried peel powder of C. kawachiensis to mice of a transient global ischemia model and found in the hippocampus region that it 1) suppressed neuronal cell death, 2) reversed the reduction in the level of phosphorylated calcium-calmodulin-dependent protein kinase II, 3) had the tendency to reverse the reduction in the level of glutathione, and 4) blocked excessive activation of microglia and astrocytes. These results suggested that the dried peel powder of C. kawachiensis had a neuroprotective effect against ischemic brain via anti-oxidative and anti-inflammatory effects. We also showed that these effects of the dried peel powder were more powerful than those obtained with a comparable amount of NGIN alone. Abstract : : rONC-TFn had a dose-dependent cytotoxicity to the HepG2 and Hela cells and incubation of L02 cells with rONC-TFn did not result in any cytotoxicity even at very high concentrations.