Ingestion of difructose anhydride III partially suppresses the deconjugation and 7α-dehydroxylation of bile acids in rats fed with a cholic acid-supplemented diet. Issue 7 (3rd July 2019)

• Record Type: Journal Article
• Authors: Lee, Dong Geun; Hori, Shota; Kohmoto, Ohji; Kitta, Shinri; Yoshida, Ryo; Tanaka, Yasutake; Shimizu, Hidehisa; Takahashi, Keisuke; Nagura, Taizo; Uchino, Hirokatsu; Fukiya, Satoru; Yokota, Atsushi; Ishizuka, Satoshi
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 83:Issue 7(2019)
• Page Start: 1329
• Page End: 1335
• Publication Date: 2019-07-03
• Subjects: Difructose anhydride III -- bile acid -- deconjugation -- 7α-dehydroxylation; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2019.1597617
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Difructose anhydride III (DFAIII) is a prebiotic involved in the reduction of secondary bile acids (BAs). We investigated whether DFAIII modulates BA metabolism, including enterohepatic circulation, in the rats fed with a diet supplemented with cholic acid (CA), one of the 12α-hydroxylated BAs. After acclimation, the rats were fed with a control diet or a diet supplemented with DFAIII. After 2 weeks, each group was further divided into two groups and was fed diet with or without CA supplementation at 0.5 g/kg diet. BA levels were analyzed in aortic and portal plasma, liver, intestinal content, and feces. As a result, DFAIII ingestion reduced the fecal deoxycholic acid level via the partial suppression of deconjugation and 7α-dehydroxylation of BAs following CA supplementation. These results suggest that DFAIII suppresses production of deoxycholic acid in conditions of high concentrations of 12α-hydroxylated BAs in enterohepatic circulation, such as obesity or excess energy intake. Abbreviation : BA: bile acid; BSH: bile salt hydrolase; CA: cholic acid; DCA: deoxycholic acid; DFAIII: difructose anhydride III; MCA: muricholic acid; MS: mass spectrometry; NCDs: non-communicable diseases; LC: liquid chromatography; SCFA: short-chain fatty acid; TCA: taurocholic acid; TCDCA: taurochenodeoxycholic acid; TDCA: taurodeoxycholic acid; TUDCA: tauroursodeoxychlic acid; TαMCA: tauro-α-muricholic acid; TβMCA: tauro-β-muricholic acid; TωMCA: tauro-ω-muricholic acid Graphical abstract: : Suppressed deconjugation and 7α-dehydroxylation of 12α-hydroxylated bile acids by difructose anhydride III contribute to reduction of deoxycholic acid.