Effects of oxygen tension and dextran-shelled/2H, 3H-decafluoropentane-cored oxygen-loaded nanodroplets on secretion of gelatinases and their inhibitors in term human placenta. Issue 3 (3rd March 2016)

• Record Type: Journal Article
• Authors: Prato, Mauro; Khadjavi, Amina; Magnetto, Chiara; Gulino, Giulia Rossana; Rolfo, Alessandro; Todros, Tullia; Cavalli, Roberta; Guiot, Caterina
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 3(2016)
• Page Start: 466
• Page End: 472
• Publication Date: 2016-03-03
• Subjects: oxygen -- nanodroplet -- matrix metalloproteinase (MMP) -- tissue inhibitor of metalloproteinase (TIMP) -- human placenta; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2015.1095068
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Matrix metalloproteinases (MMPs) and their endogenous inhibitors (TIMPs) need to be finely modulated in physiological processes. However, oxygen tension influences MMP/TIMP balances, potentially leading to pathology. Intriguingly, new 2H, 3H-decafluoropentane-based oxygen-loaded nanodroplets (OLNDs) have proven effective in abrogating hypoxia-dependent dysregulation of MMP and TIMP secretion by single cell populations. This work explored the effects of different oxygen tensions and dextran-shelled OLNDs on MMP/TIMP production in an organized and multicellular tissue (term human placenta). Chorionic villous explants from normal third-trimester pregnancies were incubated with/without OLNDs in 3 or 20% O2 . Explants cultured at higher oxygen tension released constitutive proMMP-2, proMMP-9, TIMP-1, and TIMP-2. Hypoxia significantly altered MMP-2/TIMP-2 and MMP-9/TIMP-1 ratios enhancing TIMP-2 and reducing proMMP-2, proMMP-9, and TIMP-1 levels. Intriguingly, OLNDs effectively counteracted the effects of low oxygen tension. Collectively, these data support OLND potential as innovative, nonconventional, and cost-effective tools to counteract hypoxia-dependent dysregulation of MMP/TIMP balances in human tissues. Graphical abstract: : Oxygen-loaded nanodroplets counteract hypoxia-induced dysregulation of balances between gelatinases and inhibitors in human placental explants.