Siglec-9 modulated IL-4 responses in the macrophage cell line RAW264. Issue 3 (3rd March 2016)

• Record Type: Journal Article
• Authors: Higuchi, Hiroshi; Shoji, Toru; Murase, Yusuke; Iijima, Shinji; Nishijima, Ken-ichi
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 3(2016)
• Page Start: 501
• Page End: 509
• Publication Date: 2016-03-03
• Subjects: lectin -- macrophages -- sialic acid -- IL-4 -- RAW264; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2015.1104238
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Siglecs, an immunoglobulin-like lectin family that recognizes the sialic acid moiety, regulate various aspects of immune responses. In the present study, we investigated the effects of Siglecs on the macrophage cell line RAW264, which was stimulated with interleukin-4 (IL-4). The induction of arginase-1 (Arg1) by IL-4 was stronger in Siglec-9-expressing cells than in mock cells. Mutations in the cytoplasmic tyrosine-based inhibitory motifs in Siglec-9 markedly reduced the expression of Arg1. The phosphorylation of Akt by IL-4 and extracellular signal-regulated kinase (ERK) without IL-4 was stronger in Siglec-9-expressing cells, indicating the enhanced activation of the phosphatidylinositol 3 kinase (PI-3K) and mitogen-activated protein kinase kinase (MEK)/ERK pathways, respectively. The enhanced expression of Arg1 was inhibited by MEK inhibitors, but not by PI-3K inhibitor. These results indicate that Siglec-9 affects several different signaling pathways in IL-4-stimulated macrophages, which resulted in enhanced induction of Arg1 in Siglec-9-expressing RAW264 cells. Graphical abstract: : Siglec-9-modulated IL-4 pathways in RAW264