An N-terminal region of a Myb-like protein is involved in its intracellular localization and activation of a gibberellin-inducible proteinase gene in germinated rice seeds. Issue 5 (4th May 2015)

• Record Type: Journal Article
• Authors: Sutoh, Keita; Washio, Kenji; Imai, Ryozo; Wada, Masamitsu; Nakai, Tomonori; Yamauchi, Daisuke
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 79:Issue 5(2015)
• Page Start: 747
• Page End: 759
• Publication Date: 2015-05-04
• Subjects: intracellular localization -- gibberellin -- Myb transcription factor -- rice -- seed germination; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2014.998620
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: The expression of the gene for a proteinase ( Rep1 ) is upregulated by gibberellins. The CAACTC regulatory element (CARE) of the Rep1 promoter is involved in the gibberellin response. We isolated a cDNA for a CARE-binding protein containing a Myb domain in its carboxyl-terminal region and designated the gene Carboxyl - terminal Myb1 ( CTMyb1 ). This gene encodes two polypeptides of two distinctive lengths, CTMyb1L and CTMyb1S, which include or exclude 213 N-terminal amino acid residues, respectively. CTMyb1S transactivated the Rep1 promoter in the presence of Os GAMyb, but not CTMyb1L. We observed an interaction between CTMyb1S and the rice prolamin box-binding factor (RPBF). A bimolecular fluorescence complex analysis detected the CTMyb1S and RPBF complex in the nucleus, but not the CTMyb1L and RPBF complex. The results suggest that the arrangement of the transfactors is involved in gibberellin-inducible expression of Rep1 . Abstract: : Schematic representation of the function of CTMyb1. CTMyb1S interacts with OsGAMyb in the mediation of RPBF and activate the Rep1 promoter, although CTMyb1L complexed with RPBF is exported from nucleus to cytoplasm.