Effects of phytochemicals on in vitro anti-inflammatory activity of Bifidobacterium adolescentis. Issue 5 (4th May 2015)

• Record Type: Journal Article
• Authors: Kawabata, Kyuichi; Kato, Yuri; Sakano, Taiken; Baba, Nobuyuki; Hagiwara, Kota; Tamura, Akira; Baba, Seigo; Natsume, Midori; Ohigashi, Hajime
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 79:Issue 5(2015)
• Page Start: 799
• Page End: 807
• Publication Date: 2015-05-04
• Subjects: Bifidobacterium adolescentis -- phytochemicals -- anti-inflammatory activity -- co-culture -- functional interaction; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2015.1006566
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Probiotics have been shown to improve the condition of not only the human gastrointestinal tract but also the entire body. We found that quercetin enhances the anti-inflammatory activity of Bifidobacterium adolescentis, which is abundant in human intestines. Here, we assessed whether certain phytochemicals could enhance the anti-inflammatory activity of B. adolescentis . Bifidobacteria were anaerobically cultured with phytochemicals for 3 h, and the anti-inflammatory activity of the supernatants was estimated by testing their ability to inhibit nitric oxide (NO) production by lipopolysaccharide-stimulated RAW264 macrophages. Of the 55 phytochemicals tested, phloretin, (+)-taxifolin, and (−)-epigallocatechin gallate as well as quercetin-3- O -glucoside and quercetin-4′- O -glucoside were similar to quercetin in promoting NO suppression by B. adolescentis . In addition, the phytochemicals excluding quercetin increased the concentrations of lactic and acetic acids in the co-culture supernatants. These results suggest that some phytochemicals may activate the anti-inflammatory function of B. adolescentis . Graphical abstract: : Quercetin and some phytochemicals enhance the secretion of bifidobacterial anti-inflammatory agent(s) capable of suppressing LPS-induced nitric oxide production in macrophages.