Characterization of CoPK02, a Ca2+/calmodulin-dependent protein kinase in mushroom Coprinopsis cinerea. Issue 8 (3rd August 2018)

• Record Type: Journal Article
• Authors: Yamashita, Masashi; Sueyoshi, Noriyuki; Yamada, Hiroki; Katayama, Syouichi; Senga, Yukako; Takenaka, Yasuhiro; Ishida, Atsuhiko; Kameshita, Isamu; Shigeri, Yasushi
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 82:Issue 8(2018)
• Page Start: 1335
• Page End: 1343
• Publication Date: 2018-08-03
• Subjects: Ca2+/calmodulin-dependent protein kinase -- Coprinopsis cinerea -- mushroom -- protein phosphorylation; CaM: calmodulin -- CaMK: Ca2+/calmodulin protein kinase -- GRAVY: grand average of hydropathy -- KD: kinase dead type -- MAPKAPK: mitogen-activated protein kinase-activated protein kinase -- MBP: myelin basic protein -- MLC: myosin light chain -- NJ: neighbor-joining -- PPase: protein phosphatase -- WT: wild type; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2018.1462692
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: We surveyed genome sequences from the basidiomycetous mushroom Coprinopsis cinerea and isolated a cDNA homologous to CMKA, a calmodulin-dependent protein kinase (CaMK) in Aspergillus nidulans . We designated this sequence, encoding 580 amino acids with a molecular weight of 63, 987, as CoPK02. CoPK02 possessed twelve subdomains specific to protein kinases and exhibited 43, 35, 40% identity with rat CaMKI, CaMKII, CaMKIV, respectively, and 40% identity with CoPK12, one of the CaMK orthologs in C. cinerea . CoPK02 showed significant autophosphorylation activity and phosphorylated exogenous proteins in the presence of Ca 2+ /CaM. By the CaM-overlay assay we confirmed that the C-terminal sequence (Trp346-Arg358) was the calmodulin-binding site, and that the binding of Ca 2+ /CaM to CoPK02 was reduced by the autophosphorylation of CoPK02. Since CoPK02 evolved in a different clade from CoPK12, and showed different gene expression compared to that of CoPK32, which is homologous to mitogen-activated protein kinase-activated protein kinase, CoPK02 and CoPK12 might cooperatively regulate Ca 2+ -signaling in C. cinerea . Abstract : : Growth of the basidiomycetous mushroom, Coprinopsis cinerea, is regulated by protein kinases, CoPK02, CoPK12, and CoPK32.