MADS2 regulates priming defence in postharvest peach through combined salicylic acid and abscisic acid signaling. (10th March 2022)

• Record Type: Journal Article
• Authors: Li, Chunhong; Lei, Changyi; Wang, Kaituo; Tan, Meilin; Xu, Feng; Wang, Jinsong; Zheng, Yonghua
• Editors: Vicente, Ariel
• Is Part Of: Journal of experimental botany. Volume 73:Number 11(2022)
• Page Start: 3787
• Page End: 3806
• Publication Date: 2022-03-10
• Subjects: ABA -- β-aminobutyric acid -- callose -- MADS2 -- NPR1 -- priming resistance -- Prunus persica -- Rhizopus rot; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/erac099
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract: MADS-box genes play well-documented roles in plant development, but relatively little is known regarding their involvement in defence responses. In this study, pre-treatment of peach ( Prunus persica ) fruit with β-aminobutyric acid (BABA) activated resistance against Rhizopus stolonifer, leading to a significant delay in the symptomatic appearance of disease. This was associated with an integrated defence response that included a H2 O2 burst, ABA accumulation, and callose deposition. cDNA library screening identified nucleus-localized MADS2 as an interacting partner with NPR1, and this was further confirmed by yeast two-hybrid, luciferase complementation imaging, and co-immunoprecipitation assays. The DNA-binding activity of NPR1 conferred by the NPR1–MADS2 complex was required for the transcription of SA-dependent pathogenesis-related ( PR ) and ABA-inducible CalS genes in order to gain the BABA-induced resistance, in which MAPK1-induced post-translational modification of MADS2 was also involved. In accordance with this, overexpression of PpMADS2 in Arabidopsis potentiated the transcription of a group of PR genes and conferred fungal resistance in the transgenic plants. Conversely, Arabidopsis mads2 -knockout lines showed high sensitivity to the fungal pathogen. Our results indicate that MADS2 positively participates in BABA-elicited defence in peach through a combination of SA-dependent NPR1 activation and ABA signaling-induced callose accumulation, and that this defence is also related to the post-translational modification of MADS2 by MAPK1 for signal amplification. Abstract : MADS2 regulates BABA-elicited defence against fungal rot in peach fruit through a combination of SA-dependent NPR1 activation and ABA signaling-induced callose accumulation.