Nitrate-dependent regulation of miR444-OsMADS27 signalling cascade controls root development in rice. (4th March 2022)

• Record Type: Journal Article
• Authors: Pachamuthu, Kannan; Hari Sundar, Vivek; Narjala, Anushree; Singh, Rahul R; Das, Soumita; Avik Pal, Harshith C Y; Shivaprasad, Padubidri V
• Editors: Sunkar, Ramanjulu
• Is Part Of: Journal of experimental botany. Volume 73:Number 11(2022)
• Page Start: 3511
• Page End: 3530
• Publication Date: 2022-03-04
• Subjects: Auxin -- micro RNA -- miR444 -- nitrate transporters -- OsMADS27 -- rice; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/erac083
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract : A regulatory module comprising microRNA444 and its target OsMADS27 are involved in nitrate-dependent root development by modulating the expression of key transporters, hormonal pathway genes and regulators of stress. Abstract: Nitrate is an important nutrient and a key signalling molecule for plant development. A number of transcription factors involved in the response to nitrate and their regulatory mechanisms have been identified. However, little is known about the transcription factors involved in nitrate sensing and their regulatory mechanisms among crop plants. In this study, we identified functions of a nitrate-responsive miR444:MADS-box transcription factor OsMADS27 module and its downstream targets mediating rice root growth and stress responses. Transgenic rice plants expressing miR444 target mimic improved rice root growth. Although miR444 has the potential to target multiple genes, we identified OsMADS27 as the major miR444 target that regulates the expression of nitrate transporters, as well as several key genes including expansins, and those associated with auxin signalling, to promote root growth. In agreement with this, overexpression of miRNA-resistant OsMADS27 improved root development and tolerance to abiotic stresses, while its silencing suppressed root growth. OsMADS27 mediated robust stress tolerance in plants through its ability to bind to the promoters of specific stress regulators, as observed in ChIP-seq analysis. Our results provide evidence of a nitrate-dependent miR444-OsMADS27 signalling cascade involved in the regulation of rice root growth, as well as its surprising role in stress responses.