Efficient delivery of therapeutic siRNA into glioblastoma cells using multifunctional dendrimer-entrapped gold nanoparticles. (December 2016)

• Record Type: Journal Article
• Authors: Kong, Lingdan; Wu, Yilun; Alves, Carla S; Shi, Xiangyang
• Is Part Of: Nanomedicine. Volume 12:Number 23(2017)
• Page Start: 3103
• Page End: 3115
• Publication Date: 2016-12
• Subjects: dendrimer-entrapped gold nanoparticles -- gene silencing -- RGD peptide -- siRNA; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2016-0240
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To synthesize the arginine-glycine-aspartic (RGD) functionalized dendrimer-entrapped gold nanoparticles (Au DENPs) for siRNA delivery to induce gene silencing of cancer cells in vitro and in vivo .Materials & methods: Au DENPs modified with RGD peptide via a polyethylene glycol spacer were used as a vector of two distinct small interfering RNAs (siRNAs) (VEGFvascular endothelial growth factor siRNA and B-cell lymphoma/leukemia-2 siRNA), and the physicochemical properties, cytocompatibility and transfection efficiency of Au DENP/siRNA polyplexes were characterized.Results: The Au DENP/siRNA polyplexes with good cytocompatibility and highly efficient transfection capacity can be used for the transfection of siRNAs.Conclusion: The developed functional RGD-modified Au DENPs may be used for efficient gene therapy of different types of cancer.