Serotype Distribution and Antimicrobial Resistance Profile of Haemophilus influenzae Isolated from School Children with Acute Otitis Media. (23rd May 2022)

• Record Type: Journal Article
• Authors: Safari, Dodi; Wahyono, Daniel Joko; Tafroji, Wisnu; Darmawan, Anton Budhi; Winarti, Yayah; Kusdaryanto, Wahyu Dwi; Paramaiswari, Wisiva Tofriska; Pramono, Hendro; Pratiwi, Meyta; Chamadi, Muhamad Riza
• Other Names: Ahmed Ashraf M. Academic Editor.
• Is Part Of: International journal of microbiology. Volume 2022(2022)
• Publication Date: 2022-05-23
• Subjects: Microbiology -- Periodicals; Microbiology; Microbiology -- Periodicals; Electronic journals; Periodicals; 579
• DOI: 10.1155/2022/5391291
• Languages: English
• ISSNs: 1687-918X

Abstract

Abstract : Haemophilus influenzae is a Gram-negative opportunistic bacterial pathogen of the human respiratory tract. This study describes the prevalence, serotype distribution, and susceptibility profiles of H . influenzae strains isolated from the nasopharynx of school children with acute otitis media (AOM) in Banyumas Regency, Central Java, Indonesia. H . influenzae was isolated from nasopharyngeal swab specimens using chocolate agar plates supplemented with IsoVitaleX and bacitracin. Serotyping was performed using quantitative polymerase chain reaction. Antimicrobial susceptibility profiles were determined using a microdilution broth assay. H . influenzae was present in 69.7% of samples (85/122). Nontypeable H . influenzae (NHTi) was the most common serotype (95.3%), followed by H . influenzae type b (3.5%) and H . influenzae type f (1.2%). All the H . influenzae isolates were susceptible to levofloxacin, ceftriaxone, imipenem, meropenem, cefuroxime, and cefixime. Most isolates were susceptible to sparfloxacin (99%), cefepime (99%), amoxicillin/clavulanic acid 2 : 1 (99%), ampicillin/sulbactam 2 : 1 (96%), chloramphenicol (94%), tetracycline (93%), ampicillin (87%), and clarithromycin (82%). Nineteen percent of the isolates were resistant to cotrimoxazole, and 11% of the isolates were resistant to ampicillin. This study showed that H . influenzae carriage among samples was dominated by NTHi and less susceptible to cotrimoxazole.