A polyphenolic biomacromolecule prepared from a flavonoid: Catechin as degradable microparticles. Issue 24 (8th February 2021)

• Record Type: Journal Article
• Authors: Suner, Selin Sagbas; Mohapatra, Subhra; Ayyala, Ramesh S.; Brethanabotla, Venkat R.; Sahiner, Nurettin
• Is Part Of: Journal of applied polymer science. Volume 138:Issue 24(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-02-08
• Subjects: biodegradable -- biomaterials -- biopolymers -- catechin -- renewable polymers; Polymers -- Periodicals; Polymerization -- Periodicals; 668.9
• DOI: 10.1002/app.50576
• Languages: English
• ISSNs: 0021-8995

Abstract

Abstract: Catechin (CAT) was crosslinked with trimethylolpropane triglycidyl ether (TMPTGE) to obtain degradable poly(CAT) particles in a single step. Spherical p(CAT) particles with tens of micrometer size range and an isoelectronic point at pH 1.2 were obtained. The hydrolytic degradation of p(CAT) particles provided sustainable and extended release with 264 mg/g CAT release within 10 days at pH 7.4. The antioxidant capacity of 55.0 ± 0.9 μg/ml gallic acid equivalent in terms of total phenol content, and 0.88 ± 0.3 μmol/g trolox equivalent were estimated for p(CAT) particles displaying strong radical scavenging capability. Blood clotting and hemolysis assays demonstrated dose‐dependent blood compatibility revealing higher blood compatibility for p(CAT) particles up to 10 μg/ml concentration. The cytotoxicity results show that p(CAT) particles have almost no toxicity for CCD841 normal colon cells at 250 μg/ml concentration in 24 h incubation time giving ~97% cell viability, whereas CAT molecules only provide ~34% cell viability. Abstract : The natural polyphenolic catechin (CAT) molecule possesses many bio‐beneficial properties including cell protecting, radical oxygen scavenging, prevention against various cancers, skin, lung, intestinal, pancreas, colon etc. Therefore, degradable p(CAT) microparticles releasing antioxidant CAT molecules up 10 days at physiological pH (7.4) were prepared shown to have anti‐antioxidant property and retain hemocompatibility up to 10 μg/mL concentration. Also, cell viability of p(CAT) particles revealed no toxicity against CCD841 colon cells up to 250 µg/mL.