A modern purification method for volatile sesquiterpenes produced by recombinant Escherichia coli carrying terpene synthase genes. Issue 6 (3rd June 2018)

• Record Type: Journal Article
• Authors: Shindo, Kazutoshi
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 82:Issue 6(2018)
• Page Start: 935
• Page End: 939
• Publication Date: 2018-06-03
• Subjects: Tps genes -- recombinant Escherichia coli -- purification and structural determination -- volatile sesquiterpenes -- antioxidant activity; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2017.1403882
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Most volatile sesquiterpenes had been purified from plants using distillation and preparative gas chromatography, which is not applicable to many laboratories that do not possess a needed facility. Thus, this review focuses on a modern purification method for volatile sesquiterpenes using Escherichia coli cells that functionally express terpene synthase ( Tps ) genes. It was recently developed that recombinant E. coli cells carrying Tps genes were cultured in two-layer media ( n -octane/TB medium) without harming the cells, and the volatile hydrophobic compounds trapped in the n -octane were purified by two-phase partition (alkane/alkaline 50% MeOH), silica gel column chromatography, and reversed-phase preparative high-performance liquid chromatography (if necessary). Consequently, it was found that the volatile sesquiterpenes are easily purified, the structures of which can then be determined by nuclear magnetic resonance, [α]D and gas chromatography–mass spectrometry analyses. The antioxidant activities of several volatile sesquiterpenes are also presented in this review. Abstract: : A modern purification method for volatile sesquiterpenes produced by recombinant Escherichia coli carrying terpene synthase genes.