Whey protein hydrolysates enhance water absorption in the perfused small intestine of anesthetized rats. Issue 8 (2nd August 2016)

• Record Type: Journal Article
• Authors: Ito, Kentaro; Yamaguchi, Makoto; Noma, Teruyuki; Yamaji, Taketo; Itoh, Hiroyuki; Oda, Munehiro
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 8(2016)
• Page Start: 1587
• Page End: 1593
• Publication Date: 2016-08-02
• Subjects: whey protein hydrolysates -- water absorption -- amino acid mixture -- PepT1; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2016.1166931
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: We evaluated the effect of whey protein hydrolysates (WPH) on the water absorption rate in the small intestine using a rat small intestine perfusion model. The rate was significantly higher with 5 g/L WPH than with 5 g/L soy protein hydrolysates or physiological saline ( p < 0.05). WPH dose-dependently increased the water absorption rate in the range of 1.25–10.0 g/L. WPH showed a significantly higher rate than an amino acid mixture whose composition was equal to that of WPH ( p < 0.05). The addition of 4-aminomethylbenzoic acid, an inhibitor of PepT1, significantly suppressed WPH's enhancement of water absorption ( p < 0.05). The rate of water absorption was significantly correlated with that of peptides/amino acids absorption in WPH ( r = 0.82, p < 0.01). These data suggest that WPH have a high water absorption-promoting effect, to which PepT1 contributes. Graphical abstract: : Whey protein hydrolysates promote water absorption in the rat small intestine and PepT1 is suggested to contribute to this effect.