Inhibitory effects of 3α-hydroxy-lup-20(29)-en-23, 28-dioic acid on lipopolysaccharide-induced TNF-α, IL-1β, and the high mobility group box 1 release in macrophages. Issue 7 (3rd July 2017)

• Record Type: Journal Article
• Authors: Liu, Xiang-Qian; Zou, Qin-Peng; Huang, Jian-Jun; Yook, Chang-Soo; Whang, Wan-Kyunn; Lee, Hyeong-Kyu; Kwon, Ok-Kyoung
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 81:Issue 7(2017)
• Page Start: 1305
• Page End: 1313
• Publication Date: 2017-07-03
• Subjects: 3α-hydroxy-lup-20(29)-en-23, 28-dioic acid -- anti-inflammatory -- TNF-α -- IL-1β -- HMGB1; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2017.1301803
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: We investigated the anti-inflammatory effects of 3α-hydroxy-lup-20(29)-en-23, 28-dioic acid (HLEDA)—a lupane-type triterpene isolated from leaves of Acanthopanax gracilistylus W. W.Smith (AGS), as well as the underlying molecular mechanisms in lipopolysaccharide (LPS)-induced RAW264.7 cells. Our results demonstrated that HLEDA concentration-dependently reduced the production of nitric oxide (NO), significantly suppressed LPS-induced expression of TNF-α and IL-1β at the mRNA and protein levels in RAW264.7 cells. Further analysis revealed that HLEDA could reduce the secretion of High Mobility Group Box 1 (HMGB1). Additionally, the results showed that HLEDA efficiently decreased nuclear factor-kappaB (NF-κB) activation by inhibiting the degradation and phosphorylation of IκBα. These results suggest that HLEDA exerts anti-inflammatory properties in LPS-induced macrophages, possibly through inhibition of the NF-κB signaling pathway, which mediates the expression of pro-inflammatory cytokines. These results warrant further studies that would concern candidate therapy for diseases, such as fulminant hepatitis and rheumatology of triterpenoids in AGS. Abstract : :