5, 6-Dehydrokawain from Alpinia zerumbet promotes osteoblastic MC3T3-E1 cell differentiation. Issue 7 (2nd July 2016)

• Record Type: Journal Article
• Authors: Kumagai, Momochika; Mishima, Takashi; Watanabe, Akio; Harada, Teppei; Yoshida, Izumi; Fujita, Kazuhiro; Watai, Masatoshi; Tawata, Shinkichi; Nishikawa, Keisuke; Morimoto, Yoshiki
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 7(2016)
• Page Start: 1425
• Page End: 1432
• Publication Date: 2016-07-02
• Subjects: Alpinia zerumbet -- 5, 6-dehydrokawain -- MC3T3-E1 -- osteoblast -- osteoporosis; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2016.1153959
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Bone homeostasis is maintained by balancing bone formation and bone resorption, but an imbalance between them is associated with various bone-related diseases such as osteoporosis and rheumatoid arthritis. We found that 5, 6-dehydrokawain (DK) and dihydro-5, 6-dehydrokawain (DDK), which were isolated as promising compounds from Alpinia zerumbet rhizomes, promote differentiation of osteoblastic MC3T3-E1 cells. DK and DDK increased the alkaline phosphatase activity and matrix mineralization of MC3T3-E1 cells. DK exerts larger effects than DDK. The gene expression of runt-related transcription factor 2 and osterix, which are essential transcription factors in the early period of osteoblast differentiation, was significantly increased by DK treatment. The mRNA level of distal-less homeobox 5 was also enhanced by DK treatment, and DK activated the p38 mitogen-activated protein kinase pathway. Therefore, DK may have clinical potential for preventing osteoporosis, and could be considered as a potential anabolic therapeutic agent. Graphical abstract: : 5, 6-Dehydrokawain (DK) and dihydro-5, 6-dehydrokawain (DDK) from Alpinia zerumbet rhizomes, promote differentiation of osteoblastic MC3T3-E1 cells.