Manno-oligosaccharide-binding ability of mouse RegIV/GST-fusion protein evaluated by complex formation with the carbohydrate-containing polyamidoamine dendrimer. Issue 11 (2nd November 2014)

• Record Type: Journal Article
• Authors: Kato, Yuta; Kochi, Kazunori; Unno, Hideaki; Goda, Shuichiro; Hatakeyama, Tomomitsu
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 78:Issue 11(2014)
• Page Start: 1906
• Page End: 1909
• Publication Date: 2014-11-02
• Subjects: RegIV -- polyamidoamine dendrimer -- mannose -- binding -- light scattering; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2014.940834
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: The carbohydrate-binding properties of the C-type lectin-like mouse RegIV and glutathione S-transferase-fusion protein (GST-mRegIV) were examined using carbohydrate-containing polyamidoamine dendrimers (PD). GST-mRegIV showed affinity for mannan- and manno-oligosaccharide containing PD. Binding was inhibited by manno-oligosaccharides but not by mannose or other tested carbohydrates, suggesting that the binding site may have an extended structure in contrast with typical C-type lectins.