Forced expression of FLO11 confers pellicle-forming ability and furfural tolerance on Saccharomyces cerevisiae in ethanol production. Issue 4 (3rd April 2014)

• Record Type: Journal Article
• Authors: Nakagawa, Youji; Hasebe, Toru; Ishiai, Miyuki; Yamamura, Hideki; Iimura, Yuzuru; Hayakawa, Masayuki
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 78:Issue 4(2014)
• Page Start: 714
• Page End: 717
• Publication Date: 2014-04-03
• Subjects: pellicle formation -- FLO11 -- furfural -- bioethanol -- Saccharomyces cerevisiae; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2014.895660
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: We constructed a plasmid that expresses FLO11 encoding a cell surface glycoprotein of Saccharomyces cerevisiae under the control of a constitutive promoter. This plasmid conferred pellicle-forming ability on the non-pellicle-forming industrial strain of S. cerevisiae at the air–liquid interface of the glucose-containing liquid medium. The induced pellicle-forming cells exhibited tolerance to furfural, which is a key toxin in lignocellulosic hydrolysates, in ethanol production.