Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico. Issue 1 (2nd January 2014)

• Record Type: Journal Article
• Authors: Ogawa, Takumi; Furuhashi, Takeshi; Okazawa, Atsushi; Nakai, Rai; Nakazawa, Masami; Kind, Tobias; Fiehn, Oliver; Kanaya, Shigehiko; Arita, Masanori; Ohta, Daisaku
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 78:Issue 1(2014)
• Page Start: 14
• Page End: 18
• Publication Date: 2014-01-02
• Subjects: LipidBlast -- polar lipid profiling -- LC–MS/MS -- Euglena gracilis; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2014.877826
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: A rapid protocol for polar lipid profiling was applied to Euglena gracilis lipid metabolism by LipidBlast, an MS/MS spectral similarity search tool. The similarity search results suggested anoxia-induced polar lipid metabolism in Euglena characterized by the accumulation of differential lipid classes, carbon chain lengths, and unsaturated bond numbers. The informatics-supported MS spectral search provides an alternative option for global lipid profiling studies.