Pattern analysis suggests that phosphoenolpyruvate carboxylase in maturing soybean seeds promotes the accumulation of protein. Issue 12 (2nd December 2019)

• Record Type: Journal Article
• Authors: Yamamoto, Naoki; Masumura, Takehiro; Yano, Kentaro; Sugimoto, Toshio
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 83:Issue 12(2019)
• Page Start: 2238
• Page End: 2243
• Publication Date: 2019-12-02
• Subjects: Anaplerotic -- principal component analysis -- phosphoenolpyruvate carboxylase -- seed protein -- soybean; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2019.1648205
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: The protein and oil contents in soybean seeds are major factors in seed quality. Seed proteins and oils are synthesized from sucrose and nitrogenous compounds transported into maturing seeds. In this study, we compared changes in the activity of phospho enol pyruvate carboxylase (PEPC) and the accumulation profiles of protein and oil in maturing seeds of two soybean cultivars, which exhibit different protein and oil contents in seeds, to determine the interrelationships of them. A principal component analysis indicated a concordance of seed PEPC activity with the protein content, but did not with the oil content. PEPC activity per seed was highest in the late maturation stage, when the physiological status of the vegetative organs drastically changed. The high-protein cultivar had higher PEPC activity compared to the low-protein cultivar. These results highlight the biological role of PEPC in the synthesis of protein, therefore it was implied that PEPC could be a biomarker in soybean breeding. Abbreviations: ANOVA: analysis of variance; DS: developmental stage; DW: dry weight; FW: fresh weight; NIR: near infrared; PEP(C): phospho enol pyruvate (carboxylase); PC(A): principal component (analysis); S.E.: standard error; WC: water content. Graphical abstract: : Soy seed PEPC play a role for providing carbon skelton of amino acids of storage protein from starch in responce to N supply at the late maturing stage.