Potential of extracts from Saponaria officinalis and Calendula officinalis to modulate in vitro rumen fermentation with respect to their content in saponins. Issue 2 (1st February 2014)

• Record Type: Journal Article
• Authors: Budan, Alexandre; Bellenot, Denis; Freuze, Ingrid; Gillmann, Louisa; Chicoteau, Pierre; Richomme, Pascal; Guilet, David
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 78:Issue 2(2014)
• Page Start: 288
• Page End: 295
• Publication Date: 2014-02-01
• Subjects: saponin -- rumen -- methane -- volatile fatty acid -- HPLC; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2014.882742
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Saponins have the potential to favorably modulate rumen fermentation, but there is generally a lack of the chemical structures associated with the described effects. The activity of extracts from Calendula officinalis and Saponaria officinalis in the rumen was evaluated in vitro . The S. officinalis root extract, reduced CH4 production by 8.5% and increased total VFA concentration by 25.2%. C. officinalis and S. officinalis root extracts and the S. officinalis aerial part extract decreased the acetate to propionate ratio from 8.6 to 17.4%, according to the extract. An HPLC-ELSD analysis indicated that the saponin content ranged from 43.6 to 57.6 mg/g of dry matter (DM) in the C. officinalis extracts and from 224.0 to 693.8 mg/g of DM in the S. officinalis extracts, expressed as the hederacoside C equivalent. Identification of the saponin compounds present in the extracts by HPLC–MS n suggested that the saponin profile modulated the biological activities, showing the importance of determining the structure of saponins when evaluating extracts.