Cryopreservation of the edible alkalophilic cyanobacterium Arthrospira platensis. Issue 10 (2nd October 2016)

• Record Type: Journal Article
• Authors: Shiraishi, Hideaki
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 10(2016)
• Page Start: 2051
• Page End: 2057
• Publication Date: 2016-10-02
• Subjects: cyanobacteria -- dietary supplements -- spirulina -- Arthrospira platensis -- cryopreservation; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2016.1189320
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Efficient cryopreservation conditions for the edible alkalophilic cyanobacterium Arthrospira ( Spirulina ) platensis were investigated using a model strain A. platensis NIES-39. As a result, it was found that more than 60% of cells were viable upon thawing, when they had been frozen at a cooling rate of approximately −1 °C min −1 in the presence of 10% (v/v) dimethyl sulfoxide. Further examination with other Arthrospira strains showed that many of them had strain-dependent optimal conditions for cryopreservation. For example, the best freezing conditions for A. platensis SAG 21.99 were snap-freezing in liquid nitrogen in the presence of 5% (v/v) dimethyl sulfoxide, while they were slow cooling at approximately −1 °C min −1 in the presence of 10% (v/v) methanol for A. platensis NIES-46, NIES-2308 and UTEX 1926. The variety of successful cryopreservation conditions presented in this study is useful when attempting to cryopreserve various Arthrospira strains. Graphical abstract: : The conditions for efficient cryopreservation of Arthrospira platensis were determined. They enable genetically stable preservation of many A. platensis strains.