Intracellular retention of the extracellular domain of the (pro)renin receptor in mammalian cells. Issue 7 (3rd July 2014)

• Record Type: Journal Article
• Authors: Suzuki-Nakagawa, Chiharu; Nishimura, Misa; Noda, Masako; Iwata, Hideyuki; Hattori, Masaru; Ebihara, Akio; Suzuki, Fumiaki; Nakagawa, Tsutomu
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 78:Issue 7(2014)
• Page Start: 1187
• Page End: 1190
• Publication Date: 2014-07-03
• Subjects: (pro)renin receptor -- ATP6AP2 -- extracellular domain -- phase separation -- glutathione S-transferase (GST); Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2014.915732
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: As a component of the renin-angiotensin system, the (pro)renin receptor [(P)RR] activates prorenin along with intracellular signaling pathways. In this study, the glutathione S-transferase-fused extracellular domain of (P)RR expressed in mammalian cells was recovered in the detergent phase in detergent-based two-phase separation experiments, and intracellular localization was observed by immunocytochemistry, suggesting retention inside the cell through stable membrane association.