Antidiabetic components from the hexane extract of red kidney beans (Phaseolus vulgaris L.): isolation and structure determination. Issue 3 (3rd March 2020)

• Record Type: Journal Article
• Authors: Sutedja, Anita Maya; Yanase, Emiko; Batubara, Irmanida; Fardiaz, Dedi; Lioe, Hanifah Nuryani
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 84:Issue 3(2020)
• Page Start: 598
• Page End: 605
• Publication Date: 2020-03-03
• Subjects: Red kidney beans -- antidiabetic -- α-glucosidase inhibition -- triacylglycerol; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2019.1691911
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Red kidney beans ( Phaseolus vulgaris L.) contain bioactive compounds that are known to exhibit antidiabetic effects via inhibition of α-glucosidase. However, information on the nonpolar components that exhibit antidiabetic activity is limited. Here, we report the isolation and structure determination of components with α-glucosidase inhibitory activity, which were obtained from the hexane extract of red kidney beans. Triacylglycerols (TAGs) were identified as the major components exhibiting inhibitory activity against α-glucosidase. The chemical structure of TAGs was determined by a combination of GC-MS and UPLC-MS/MS. The primary TAGs identified were LnLnLn (trilinolenin) and LnLLn (1, 3-dilinolenoyl-2-linoleoyl glycerol). The major fatty acids present in these TAGs were α-linolenic acid (ω-3) and linoleic acid (ω-6). These TAGs were also found to inhibit the α-glucosidase activity in a similar fashion as acarbose. These results suggest that TAGs have potency as antidiabetics and support the potential suitability of red kidney beans for diabetes treatment. Graphical abstract: : The dominant lipophilic compound of red kidney bean were identified as triacylglycerol and known to have α-glucosidase inhibition activity