Determination by UPLC-MS of four dammarane-type saponins from heat-processed Gynostemma pentaphyllum. Issue 2 (1st February 2014)

• Record Type: Journal Article
• Authors: Wu, Qian; Jang, Moonhee; Piao, Xiang-Lan
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 78:Issue 2(2014)
• Page Start: 311
• Page End: 316
• Publication Date: 2014-02-01
• Subjects: Gynostemma pentaphyllum -- gypenoside L -- gypenoside LI -- damulin B -- damulin A; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2014.882751
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Heat-processed Gynostemma pentaphyllum and its main dammaran-type saponins, gypenoside L, gypenoside LI, damulin B, and damulin A, possess non-small cell lung carcinoma A549 cell inhibitory activity. We established in this study a method by ultra-high performance liquid chromatography with tandem mass spectrometry for determination of the saponins and also investigated their content change in heat-processed G. pentaphyllum . The main saponins increased with increasing heating temperature and time. Further investigation showed that they were produced from gypenoside XLVI and gypenoside LVI by undergoing hydrolysis during the heat treatment.