Silencing of lncRNA ZFAS1 inhibits malignancies by blocking Wnt/β-catenin signaling in gastric cancer cells. Issue 3 (4th March 2018)

• Record Type: Journal Article
• Authors: Xu, Weiran; He, Liang; Li, Ying; Tan, Yan; Zhang, Fan; Xu, Hong
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 82:Issue 3(2018)
• Page Start: 456
• Page End: 465
• Publication Date: 2018-03-04
• Subjects: Cell cycle -- epithelial-mesenchymal transition -- gastric cancer -- lncRNA -- Wnt/β-catenin signaling; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2018.1431518
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Gastric cancer is a common malignancy with high mortality. Long noncoding RNA (lncRNA) zinc finger antisense (ZFAS)1 is upregulated in gastric cancer specimens compared with the para-carcinoma tissues. The silencing of ZFAS1 inhibited the growth, proliferation, cell cycle progress, migration, invasion and epithelial-mesenchymal transition (EMT), and enhanced the sensitivity to cis-platinum or paclitaxel in SGC7901 cells, as evidenced by the expression changes of proliferating cell nuclear antigen, Cyclin D1, Cyclin E, Cyclin B1, E-cadherin, N-cadherin, vimentin, matrix metalloproteinase (MMP)-2 and MMP-14. The ZFAS1 also activated the Wnt/β-catenin signaling. Subsequently, the ZFAS1 knockdown-induced the inhibition of migration, invasion, EMT and resistance to chemotherapeutic reagens was reversed by the overexpression of β-catenin. In summary, the silencing of ZFAS1 inhibited the growth, proliferation, cell cycle progress, migration, invasion, EMT and chemotherapeutic tolerance by blocking the Wnt/β-catenin signaling in gastric cancer cells. Abstract: : ZFAS1 knockdown decreases the nuclear level of β-catenin (A). Ectopic expression of β-catenin enhances EMT in ZFAS1-silenced SGC7901 cells (B).