Diosgenin effectively suppresses skin inflammation induced by phthalic anhydride in IL-4/Luc/CNS-1 transgenic mice. Issue 5 (3rd May 2016)

• Record Type: Journal Article
• Authors: Kim, Ji Eun; Go, Jun; Koh, Eun Kyoung; Song, Sung Hwa; Sung, Ji Eun; Lee, Hyun Ah; Kim, Dong Seob; Son, Hong Joo; Lee, Hee Seob; Lee, Chung Yeoul; Hong, Jin Tae; Hwang, Dae Youn
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 5(2016)
• Page Start: 891
• Page End: 901
• Publication Date: 2016-05-03
• Subjects: diosgenin -- skin inflammation -- IL-4/Luc/CNS-1 transgenic mice -- phthalic anhydride -- IgE; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2015.1135040
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: To quantitatively evaluate the therapeutic effects of diosgenin (DG) and investigate the role of IL-4 on skin inflammation, alterations in luciferase-derived signal and general phenotype biomarkers were measured in IL-4/Luc/CNS-1 transgenic mice with phthalic anhydride (PA)-induced skin inflammation after treatment with DG for 4 weeks. High levels of luciferase-derived signal detected in the abdominal region and submandibular lymph node (SL) of the PA treated group was significantly decreased by 67–88% in the PA + DG cotreated group. Furthermore, the weight of the lymph node and spleen, IgE concentration, epidermis thickness, and number of infiltrated mast cells were lower in the PA + DG treated group than the PA + Vehicle treated group. Moreover, expression of IL-6 and vascular endothelial growth factor (VEGF) also decreased in the PA + DG cotreated group. These results suggest that PA-induced skin inflammation could be successfully suppressed by DG treatment in IL-4/Luc/CNS-1 Tg mice through attenuation of IL-4 and IL-6 expression, as well as decreased IgE concentration and mast cells infiltration. Graphical abstract: : Measurement of luciferase signal in the whole body (A) and each organ (B) of IL-4/Luc/CNS-1 Tg mice.