Structure-based design and synthesis of a bivalent iminobiotin analog showing strong affinity toward a low immunogenic streptavidin mutant. Issue 4 (3rd April 2015)

• Record Type: Journal Article
• Authors: Kawato, Tatsuya; Mizohata, Eiichi; Shimizu, Yohei; Meshizuka, Tomohiro; Yamamoto, Tomohiro; Takasu, Noriaki; Matsuoka, Masahiro; Matsumura, Hiroyoshi; Kodama, Tatsuhiko; Kanai, Motomu; Doi, Hirofumi; Inoue, Tsuyoshi; Sugiyama, Akira
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 79:Issue 4(2015)
• Page Start: 640
• Page End: 642
• Publication Date: 2015-04-03
• Subjects: streptavidin -- iminobiotin -- bivalency -- X-ray structural analysis -- pre-targeting; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2014.991692
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: The streptavidin/biotin interaction has been widely used as a useful tool in research fields. For application to a pre-targeting system, we previously developed a streptavidin mutant that binds to an iminobiotin analog while abolishing affinity for natural biocytin. Here, we design a bivalent iminobiotin analog that shows 1000-fold higher affinity than before, and determine its crystal structure complexed with the mutant protein.