Further validation of unsaturated long-chain fatty acids as inhibitors for oxidized low-density lipoprotein binding to CD36 via assays with synthetic CD36 peptide-cross-linked plates. Issue 5 (4th May 2014)

• Record Type: Journal Article
• Authors: Kozai, Yuki; Tsuzuki, Satoshi; Takai, Marie; Eguchi, Ai; Matsumura, Shigenobu; Inoue, Kazuo; Fushiki, Tohru
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 78:Issue 5(2014)
• Page Start: 839
• Page End: 842
• Publication Date: 2014-05-04
• Subjects: binding inhibition -- CD36 -- oxidized low-density lipoproteins -- peptide cross linking -- unsaturated long-chain fatty acids; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2014.891934
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: We recently obtained evidence that unsaturated long-chain fatty acids (LCFAs) (e.g. oleic acid) inhibit binding of oxidized low-density lipoproteins (oxLDLs) to CD36. In the present study, we validated this prediction by examining inhibition by unsaturated LCFAs of Alexa-fluor-labeled oxLDL binding to multiwell plates onto which a synthetic CD36 peptide is covalently immobilized via thiol–maleimide coupling.