MPK9 and MPK12 function in SA-induced stomatal closure in Arabidopsis thaliana. Issue 7 (3rd July 2017)

• Record Type: Journal Article
• Authors: Khokon, Md Atiqur Rahman; Salam, Mohammad Abdus; Jammes, Fabien; Ye, Wenxiu; Hossain, Mohammad Anowar; Okuma, Eiji; Nakamura, Yoshimasa; Mori, Izumi C; Kwak, June M; Murata, Yoshiyuki
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 81:Issue 7(2017)
• Page Start: 1394
• Page End: 1400
• Publication Date: 2017-07-03
• Subjects: cytosolic alkalization -- mitogen-activated protein kinase -- reactive oxygen species -- salicylic acid -- S-type anion channel in guard cells; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2017.1308244
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Salicylic acid (SA) induces stomatal closure sharing several components with abscisic acid (ABA) and methyl jasmonate (MeJA) signaling. We have previously shown that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signaling and MeJA signaling in Arabidopsis thaliana . In this study, we examined whether these two MAPKs are involved in SA-induced stomatal closure using genetic mutants and a pharmacological, MAPKK inhibitor. Salicylic acid induced stomatal closure in mpk9 and mpk12 single mutants but not in mpk9 mpk12 double mutants. The MAPKK inhibitor PD98059 inhibited SA-induced stomatal closure in wild-type plants. Salicylic acid induced extracellular reactive oxygen species (ROS) production, intracellular ROS accumulation, and cytosolic alkalization in the mpk9, mpk12, and mpk9 mpk12 mutants. Moreover, SA-activated S-type anion channels in guard cells of wild-type plants but not in guard cells of mpk9 mpk12 double mutants. These results imply that MPK9 and MPK12 are positive regulators of SA signaling in Arabidopsis guard cells. Abstract : :