Tumor cell-derived secretory factor downregulates Semaphorin-3a in osteoblasts by activating mammalian target of rapamycin pathway. Issue 5 (3rd May 2016)

• Record Type: Journal Article
• Authors: Yamada, Daisuke; Kawahara, Kohichi; Ozaki, Masanobu; Maeda, Takehiko
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 5(2016)
• Page Start: 942
• Page End: 944
• Publication Date: 2016-05-03
• Subjects: osteoblast -- Semaphorin-3a -- mTORC1; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2015.1136881
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: We found that conditioned medium derived from Lewis Lung Carcinoma cells down-regulated Semaphorin3a ( Sema3a ) mRNA expression and increased the activity of mammalian target of rapamycin complex 1 (mTORC1) in osteoblast-like MC3T3-E1 cells. Furthermore, mTORC1 inhibition with rapamycin counteracted the effect of conditioned media on Sema3a mRNA expression. These results suggest that tumor cells decrease Sema3a mRNA expression in osteoblast in an mTORC1-dependent manner.