Constitutively expressed Siglec-9 inhibits LPS-induced CCR7, but enhances IL-4-induced CD200R expression in human macrophages. Issue 6 (2nd June 2016)

• Record Type: Journal Article
• Authors: Higuchi, Hiroshi; Shoji, Toru; Iijima, Shinji; Nishijima, Ken-ichi
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 6(2016)
• Page Start: 1141
• Page End: 1148
• Publication Date: 2016-06-02
• Subjects: human -- lectin -- macrophages -- sialic acid -- Siglec; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2016.1146070
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Siglecs recognize the sialic acid moiety and regulate various immune responses. In the present study, we compared the expression levels of Siglecs in human monocytes and macrophages using a quantitative real-time reverse transcription–polymerase chain reaction analysis. The differentiation of monocytes into macrophages by macrophage colony-stimulating factor or granulocyte macrophage colony-stimulating factor enhanced the expression of Siglec-7 and Siglec-9. The differentiated macrophages were stimulated by lipopolysaccharide (LPS) plus interferon (IFN)-γ or interleukin (IL)-4. The expression of Siglec-10 was enhanced by IL-4, whereas that of Siglec-7 was reduced by LPS plus IFN-γ. The expression of Siglec-9 was not affected by these stimuli. The knockdown of Siglec-9 enhanced the expression of CCR7 induced by the LPS or the LPS plus IFN-γ stimulation, and decreased the IL-4-induced expression of CD200R. These results suggest that Siglec-9 is one of the main Siglecs in human blood monocytes/macrophages and modulates innate immunity. Graphical abstract: : Siglec-9 modulated immune responses in human macrophages.