Loss of ATP-dependent lysine uptake in the vacuolar membrane vesicles of Saccharomyces cerevisiae ypq1∆ mutant. Issue 7 (3rd July 2014)

• Record Type: Journal Article
• Authors: Sekito, Takayuki; Nakamura, Kyosuke; Manabe, Kunio; Tone, Junichi; Sato, Yumika; Murao, Nami; Kawano-Kawada, Miyuki; Kakinuma, Yoshimi
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 78:Issue 7(2014)
• Page Start: 1199
• Page End: 1202
• Publication Date: 2014-07-03
• Subjects: vacuole -- transport -- lysine -- PQ-loop; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2014.918489
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Saccharomyces cerevisiae Ypq1p is a vacuolar membrane protein of the PQ-loop protein family . We found that ATP-dependent uptake activities of amino acids by vacuolar membrane vesicles were impaired by ypq1 ∆ mutation. Loss of lysine uptake was most remarkable, and the uptake was recovered by overproduction of Ypq1p. Ypq1p is thus involved in transport of amino acids into vacuoles.