Single-laboratory validation for the determination of caffeic acid and seven caffeoylquinic acids in sweet potato leaves. Issue 12 (2nd December 2014)

• Record Type: Journal Article
• Authors: Sasaki, Kazunori; Oki, Tomoyuki; Kobayashi, Toru; Kai, Yumi; Okuno, Shigenori
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 78:Issue 12(2014)
• Page Start: 2073
• Page End: 2080
• Publication Date: 2014-12-02
• Subjects: caffeoylquinic acid -- sweet potato leaves -- HPLC -- single-laboratory validation; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2014.942253
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: A single-laboratory validation study was conducted on an HPLC method for the detection and quantification of caffeic acid (CA) and seven species of caffeoylquinic acids (CQAs) in lyophilized sweet potato leaves. The procedure for extraction of the analytes from the matrix and the HPLC conditions for the efficient separation of CA and CQAs were optimized. In the proposed method, a relative response factor to one of the CQAs (5-CQA) was used to quantify the others. The method performed well in terms of precision when carried out on five different days and demonstrated Horwitz ratio (HorRat) scores ranging from 0.5 to 1.0 for all analytes, which were well within the limits of performance acceptability. Accuracy testing at three levels showed an overall recovery of 94% when duplicated on five different days. Moreover, a stability study revealed that all analytes in both standard solution and sample extract were stable for 28 days. Graphical abstract: : Main contents of the article titled "Single-laboratory validation for the determination of caffeic acid and seven caffeoylquinic acids in sweet potato leaves"