Two G-box–like elements essential to high gene expression of SlAKR4B in tomato leaves. Issue 3 (4th March 2018)

• Record Type: Journal Article
• Authors: Suekawa, Marina; Fujikawa, Yukichi; Esaka, Muneharu
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 82:Issue 3(2018)
• Page Start: 425
• Page End: 432
• Publication Date: 2018-03-04
• Subjects: Aldo-keto reductase -- G-box–like element -- promoter activity -- Solanum lycopersicum -- stress response; AKR: aldo-keto reductase -- nt: nucleotide -- SA: salicylic acid -- JA: jasmonic acid -- EC: electrical conductivity; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2018.1429887
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Aldo-keto reductases (AKRs) play important roles in aldehyde detoxification as well as primary and secondary metabolism in plants. We previously reported inducible expression of a Solanum lycopersicum AKR4B ( SlAKR4B ) in tomato leaves treated with salicylic acid and jasmonic acid, and high promoter activity of SlAKR4B in tomato leaf protoplasts. In this study, we investigated the expression response of SlAKR4B in the tomato leaves with infiltration treatment and the cis -element(s) involved in high promoter activity. Gene expression analysis in tomato leaf protoplasts and buffer-infiltrated tomato leaves suggested that cell damage caused the increased expression of SlAKR4B . Promoter activity of SlAKR4B was significantly reduced by mutation of two G–box like elements. It is suggested that the two G–box like elements are responsible for the high promoter activity. Abstract: : The two G-box–like elements located at –585 to –582 bp and –510 to –507 bp of SlAKR4B promoter are essential to high promoter activity.