Production of p-Aminobenzoic acid by metabolically engineered Escherichia coli. Issue 2 (1st February 2014)

• Record Type: Journal Article
• Authors: Koma, Daisuke; Yamanaka, Hayato; Moriyoshi, Kunihiko; Sakai, Kiyofumi; Masuda, Takaya; Sato, Yoshihiro; Toida, Kozo; Ohmoto, Takashi
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 78:Issue 2(2014)
• Page Start: 350
• Page End: 357
• Publication Date: 2014-02-01
• Subjects: metabolic engineering -- para-aminobenzoic acid -- aromatic compound -- Escherichia coli; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2014.878222
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: The production of chemical compounds from renewable resources is an important issue in building a sustainable society. In this study, Escherichia coli was metabolically engineered by introducing T7lac promoter-controlled aroF fbr, pabA, pabB, and pabC genes into the chromosome to overproduce para -aminobenzoic acid (PABA) from glucose. Elevating the copy number of chromosomal PT7la c -pabA - pabB distinctly increased the PABA titer, indicating that elevation of 4-amino-4-deoxychorismic acid synthesis is a significant factor in PABA production. The introduction of a counterpart derived from Corynebacterium efficiens, pabAB (ce), encoding a fused PabA and PabB protein, resulted in a considerable increase in the PABA titer. The introduction of more than two copies of PT7la c -pabAB (ce-mod), a codon-optimized pabAB (ce), into the chromosome of a strain that simultaneously overexpressed aroF fbr and pabC resulted in 5.1 mM PABA from 55.6 mM glucose (yield 9.2%). The generated strain produced 35 mM (4.8 g L −1 ) PABA from 167 mM glucose (yield 21.0%) in fed-batch culture. Graphical abstract: : Escherichia coli was metabolically engineered by introducing several genes into the chromosome to overproduce para -aminobenzoic acid from glucose.