High levels of expression of multiple enzymes in the Smirnoff-Wheeler pathway are important for high accumulation of ascorbic acid in acerola fruits. Issue 9 (2nd September 2019)

• Record Type: Journal Article
• Authors: Suekawa, Marina; Fujikawa, Yukichi; Inoue, Akari; Kondo, Takayuki; Uchida, Eriko; Koizumi, Takeshi; Esaka, Muneharu
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 83:Issue 9(2019)
• Page Start: 1713
• Page End: 1716
• Publication Date: 2019-09-02
• Subjects: acerola -- ascorbic acid -- multiple expressions -- the Smirnoff-Wheeler pathway; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2019.1608808
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Acerola fruits contain abundant ascorbic acid (AsA). The gene expression levels of three upstream enzymes in the primary AsA biosynthesis pathway were correlated with AsA contents in the fruits of two acerola cultivars. Multiple overexpression of the enzymes increased AsA contents, suggesting their high expression is important for high AsA accumulation in acerola fruits and the breeding of AsA-rich plants. Abbreviations: AsA: ascorbic acid; PMI: phosphomannose isomerase; PMM: phosphomannomutase; GMP: GDP-d -mannose pyrophosphorylase; GME: GDP-d -mannose 3ʹ, 5ʹ-epimerase; GGP: GDP-l -galactose phosphorylase; GPP: l -galactose-1-phosphate phosphatase; GDH: l -galactose dehydrogenase; GLDH: l -galactono-1, 4-lactone dehydrogenase