Effect and mechanism of mGluR6 on the biological function of rat embryonic neural stem cells. Issue 6 (3rd June 2019)

• Record Type: Journal Article
• Authors: Zhang, Jing; Zhang, Lu; Chen, Yani; Shi, Haiyan; Huang, Xiaoyong; Wang, Yanfeng; Wang, Yu; Wei, Yameng; Xue, Wanjuan; Han, Jiming
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 83:Issue 6(2019)
• Page Start: 1027
• Page End: 1034
• Publication Date: 2019-06-03
• Subjects: Neural stem cells (NSCs) -- Metabotropic glutamate receptor 6 (mGluR6) -- cell proliferation -- apoptosis; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2019.1578639
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Here, we investigated the effects and molecular mechanisms of metabotropic glutamate receptor 6 (mGluR6) on rat embryonic neural stem cells (NSCs). Overexpression of mGluR6 significantly promoted the proliferation of NSCs and increased the diameter of neutrospheres after treatment for 24 h, 48 h and 72 h. Overexpression of mGluR6 promoted G1 to S phase transition, with significantly decreased cell ratio in G1/G0 phase but significantly increased cell ratio in S phase. Additionally, mGluR6 overexpression for 48 h decreased the early and late apoptosis significantly. Moreover, overexpression of mGluR6 significantly increased the expression of p-ERK1/2, Cyclin D1 and CDK2, while the expression of p-p38 was significantly decreased. On the contrary, these effects of mGluR6 overexpression were reversed by mGluR6 knockdown. In conclusion, mGluR6 promotes the proliferation of NSCs by activation of ERK1/2-Cyclin D1/CDK2 signaling pathway and inhibits the apoptosis of NSCs by blockage of the p38 MAPK signaling pathway. Graphical abstract: : mGluR6 promotes the proliferation of NSCs via ERK1/2-Cyclin D1/CDK2 pathway and inhibits apoptosis of NSCs by blockage of the p38 MAPK pathway.