Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish. Issue 12 (2nd December 2019)

• Record Type: Journal Article
• Authors: Ikeda, Takeshi; Ichikawa, Kohei; Shigeto, Hajime; Ishida, Takenori; Hirota, Ryuichi; Funabashi, Hisakage; Kuroda, Akio
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 83:Issue 12(2019)
• Page Start: 2272
• Page End: 2275
• Publication Date: 2019-12-02
• Subjects: Arginine -- cell dissociation -- cell sheet fabrication -- polystyrene culture dish -- tissue culture polystyrene; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2019.1659716
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Here, we report a novel non-enzymatic cell dissociation method, based on our finding that adherent cells dissociate rapidly from the polystyrene culture dish when incubated in an l - or d -arginine-containing solution. We also demonstrate the successful detachment of confluent NIH/3T3 cell monolayers from the culture dish as a cell sheet by the addition of an arginine solution.