Dense array of floating water droplets aligned to an assembly of tubular wells. (8th February 2021)

• Record Type: Journal Article
• Authors: Shimazaki, Yuzuru; Shiratori, Akiko; Harada, Kunio; Nakagawa, Tatsuo; Tanaka, Junko; Uematsu, Chihiro
• Is Part Of: Japanese journal of applied physics. Volume 60:Number 3(2021)
• Publication Date: 2021-02-08
• Subjects: floating droplets -- digital PCR -- melting temperature of DNA; Physics -- Periodicals; 621.05
• DOI: 10.35848/1347-4065/abdc37
• Languages: English
• ISSNs: 0021-4922

Abstract

Abstract: A dense array of surfactant-stabilized water droplets (20 μ m in diameter) floating on the surface of fluorinated oil was prepared. More than 30000 droplets were planarly arrayed in an 8 mm squared area using the "droplet guide", an assembly of tubular wells located on the top wall of the fluidic channel. Parallel measurement of the melting temperatures of digitally partitioned KRAS DNAs hybridized with molecular beacons was demonstrated using the array of floating droplets.