Observing DNA in live cells. (19th June 2018)

• Record Type: Journal Article
• Authors: Weiss, Lucien E.; Naor, Tal; Shechtman, Yoav
• Is Part Of: Biochemical Society transactions. Volume 46:Number 3(2018)
• Page Start: 729
• Page End: 740
• Publication Date: 2018-06-19
• Subjects: CRISPR -- DNA -- fluorescence -- microscopy -- single molecule -- super resolution; Biochemistry -- Congresses; 572
• DOI: 10.1042/BST20170301
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : The structural organization and dynamics of DNA are known to be of paramount importance in countless cellular processes, but capturing these events poses a unique challenge. Fluorescence microscopy is well suited for these live-cell investigations, but requires attaching fluorescent labels to the species under investigation. Over the past several decades, a suite of techniques have been developed for labeling and imaging DNA, each with various advantages and drawbacks. Here, we provide an overview of the labeling and imaging tools currently available for visualizing DNA in live cells, and discuss their suitability for various applications.