Exploring the combinatorial space of complete pathways to chemicals. (19th June 2018)

• Record Type: Journal Article
• Authors: Wang, Lin; Ng, Chiam Yu; Dash, Satyakam; Maranas, Costas D.
• Is Part Of: Biochemical Society transactions. Volume 46:Number 3(2018)
• Page Start: 513
• Page End: 522
• Publication Date: 2018-06-19
• Subjects: de novo pathway -- MILP -- pathway design -- stoichiometry; Biochemistry -- Congresses; 572
• DOI: 10.1042/BST20170272
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : Computational pathway design tools often face the challenges of balancing the stoichiometry of co-metabolites and cofactors, and dealing with reaction rule utilization in a single workflow. To this end, we provide an overview of two complementary stoichiometry-based pathway design tools optStoic and novoStoic developed in our group to tackle these challenges. optStoic is designed to determine the stoichiometry of overall conversion first which optimizes a performance criterion (e.g. high carbon/energy efficiency) and ensures a comprehensive search of co-metabolites and cofactors. The procedure then identifies the minimum number of intervening reactions to connect the source and sink metabolites. We also further the pathway design procedure by expanding the search space to include both known and hypothetical reactions, represented by reaction rules, in a new tool termed novoStoic. Reaction rules are derived based on a mixed-integer linear programming (MILP) compatible reaction operator, which allow us to explore natural promiscuous enzymes, engineer candidate enzymes that are not already promiscuous as well as design de novo enzymes. The identified biochemical reaction rules then guide novoStoic to design routes that expand the currently known biotransformation space using a single MILP modeling procedure. We demonstrate the use of the two computational tools in pathway elucidation by designing novel synthetic routes for isobutanol.