Varietal differences in flavonoid and antioxidant activity in Japanese soybean accessions. Issue 4 (10th December 2020)

• Record Type: Journal Article
• Authors: Arifin, Hana Akari; Hashiguchi, Takuyu; Nagahama, Kiyoko; Hashiguchi, Masatsugu; Muguerza, Melody; Sakakibara, Yoichi; Tanaka, Hidenori; Akashi, Ryo
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 85:Issue 4(2021)
• Page Start: 916
• Page End: 922
• Publication Date: 2020-12-10
• Subjects: antioxidant activity -- flavonoid -- Glycine max; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1093/bbb/zbaa104
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Soybean seeds contain many antioxidants, including flavonoids and other phytochemicals. Isoflavone is a phytoestrogen that mimics estrogenic effects on target tissues and also exerts antioxidant activity by sequestering free radicals. Despite many cultivars developed to date, varietal differences in flavonoid content and antioxidant activity in Japanese soybean accessions remain less well characterized. Here, we evaluated the seed content of isoflavones, total flavonoids, and total phenolics in 26 soybean accessions. Next, the antioxidant activity of each accession was examined using antioxidant responsive element (ARE) linked to a luciferase reporter in human HepG2 stable cells. The relative ARE luciferase activity rate of all soybean accessions varied up to 4-fold which ranged from 1.00 to 4.02; and 22 accessions exhibited significant antioxidant activities. Correlation analysis indicated that the level of total isoflavone moderately correlated with antioxidant activity. Graphical Abstract: