Ex vivo potential of a quinoline-derivative nail lacquer as a new alternative for dermatophytic onychomycosis treatment. Issue 3 (27th March 2021)

• Record Type: Journal Article
• Authors: Machado, Gabriella da Rosa Monte; Pippi, Bruna; Berlitz, Simone; Diedrich, Denise; Defferrari, Diego; Lopes, William; Gnoatto, Simone Cristina Baggio; Kulkamp-Guerreiro, Irene Clemes; Vainstein, Marilene Henning; Jean, Mickael; Van de Weghe, Pierre; de Andrade, Saulo Fernandes; Fuentefria, Alexandre Meneghello
• Is Part Of: Journal of medical microbiology. Volume 70:Issue 3(2021)
• Publication Date: 2021-03-27
• Subjects: onychomycosis -- dermatophytes -- ex vivomodel -- fungicidal effect -- quinoline derivative; Medical microbiology -- Periodicals; 616.9041
• DOI: 10.1099/jmm.0.001314
• Languages: English
• ISSNs: 0022-2615

Abstract

Abstract : Introduction. Onychomycosis infections currently show a significant increase, affecting about 10 % of the world population. Trichophyton rubrum is the main agent responsible for about 80 % of the reported infections. The clinical cure for onychomycosis is extremely difficult and effective new antifungal therapy is needed. Hypothesis/Gap Statement. Ex vivo onychomycosis models using porcine hooves can be an excellent alternative for evaluating the efficacy of new anti-dermatophytic agents in a nail lacquer. Aim. Evaluation of the effectiveness of a nail lacquer containing a quinoline derivative on an ex vivo onychomycosis model using porcine hooves, as well as the proposal of a plausible antifungal mechanism of this derivative against dermatophytic strains. Methodology. The action mechanism of a quinoline derivative was evaluated through the sorbitol protection assay, exogenous ergosterol binding, and the determination of the dose-response curves by time-kill assay. Scanning electron microscopy evaluated the effect of the derivative in the fungal cells. The efficacy of a quinoline-derivative nail lacquer on an ex vivo onychomycosis model using porcine hooves was evaluated as well. Results. The quinoline derivative showed a time-dependent fungicidal effect, demonstrating reduction and damage in the morphology of dermatophytic hyphae. In addition, the ex vivo onychomycosis model was effective in the establishment of infection by T. rubrum . Conclusion. Treatment with the quinoline-derivative lacquer showed a significant inhibitory effect on T. rubrum strain in this infection model. Finally, the compound presents high potential for application in a formulation such as nail lacquer as a possible treatment for dermatophytic onychomycosis.